Ali and Ali's Sister
From Homestar Runner Wiki
Ali and her sister were two girls featured in the email sisters who wanted to be Strong Bad's girlfriend. He imagines Ali as a blonde and her sister as a brunette. Unfortunately, Strong Bad's malfunctioning Tandy 400 deleted their email before Strong Bad even had a chance to reply. They have since made a few appearances around the site, as Strong Bad cherishes their memory. He always wished he could do over that email. Bubs also made a Grape-Nuts Robot resembling Strong Bad in personal favorites that could say "Come back Ali. Come back Ali's sister", a quote from the email in which they first appeared.
The images of Ali and her sister were taken from the video game Leisure Suit Larry 1: In the Land of the Lounge Lizards.