Homestar Runner's Daisy Dukes
From Homestar Runner Wiki
The Daisy Dukes were very short purple pants worn by Homestar Runner in the email long pants. After Strong Bad edited an email to say "creepy pants all the time get some," Homestar put them on in the hopes of getting some "light globes." Strong Bad was very disturbed by them. After they fell off and Homestar ran away, The Cheat burned them with the BMW Lighter, incinerating them in a puff of green smoke.
In the Character's Commentary for long pants, it's suggested that the pair of shorts was originally one of Marzipan's skirts that Homestar stole and dyed purple, leading him to explain to her that he "doesn't wear her skirts anymore".
Homestar again put on some Daisy Dukes (blue ones this time) as part of his Tobias Funke costume in I Killed Pom Pom.